Well, this post is gonna to be a bit bored. I have finally start my final year project with the enumeration of yoghurt mix culture. I did not expect the lab work which i am going to deal with is more or so related to microbiology, that is dealing with bacteria!!
While it may sound easy to prepare the MRS agar and M17 agar for culturing probiotic bacteria, well,in reality it is not! Put aside the need to wait for the autoclave of apparatus and agar for 1 hour, we need to be very extra careful with the agar so that it won't solidify while being left to cool down after autoclave.
Guess what! it takes my supervisor and I triple work to be able to get the reason we are unable to see the grow of bacteria colonies in the agar plate. The culprit is EXPIRED MRS agar! Too bad we weren't notice that! The expired MRS agar is dark brown colour while we were comparing it with the unexpired one which is light brown colour.
So, last friday, i was staying in lab from 11am till 7.30pm again to repeat my bacteria culturing. hopefully my 3rd time trial will be succeed! Can't wait to see the growth of probiotic bacteria on Tuesday!=)
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| I am bored with this autoclave--After long wait and repeated use! |
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| Everything will be extremely sterile inside Vertical Laminar Flow (VLF) |
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| After autoclave the Agar and Peptone water |
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| Serial dilution of sanple with peptone water done in the VLF |
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| The ready innoculated agar waited to be solidified! |
I definitely need passion and patience in order to carry out my lab work! there are still lots more to go!
